Valorization of Spent Sugarcane Fermentation Broth as a Source of Phenolic Compounds

A methodology based on a solid phase extraction (SPE) was optimized for the recovery of phenolic compounds from spent fermentation broth generated Biofene® (trans-?-farnesene) production. For this purpose, two resins (XAD-2 and HP-20) three desorption solutions (water, 50/50 ethanol/water, ethanol) were tested. The most efficient resin revealed to be HP-20, using ethanol as solution, reaching an overall total compound ca. 80% when 6 BV (bed volume) both feed applied. optimization resin’s process cycle pointed 15 treated per same volume in step, with no need extra regeneration stably yielding 48% at least 4 cycles, translating into 60 being resin, still perfectly active. extract characterized LC?ESI?UHR?QqTOF?MS, 82 identified, negative positive ionization modes, respectively. Organic acids main class identified phenolic-rich extract, followed by compounds, saccharides, peptides or amino vitamins. Additionally, significant antioxidant capacity (914.1 ± 51.6 2764.5 142.8 µmol Trolox equivalents/g-dw, respectively, ABTS ORAC methodologies), which might interesting wide variety applications.